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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B FC

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up-regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).
Catalog #	T8050-13
Applications	Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested.
Recommended Dilutions	Western Blot: 4-6ug/ml
	Flow Cytometry (Cell Surface): 1ug labels 10e6 cells
	Optimal dilutions to be determined by the researcher.
Positive Control	Liver
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.05% sodium azide.
Purity	Purified by Protein G chromatography
Immunogen	Synthetic peptide corresponding to aa375-390 of mouse TLR2.
Specificity	Recognizes mouse TLR2. Species Crossreactivity: rat, human and chimpanzee.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.