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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH FC

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 cDNA codes for a protein with approximate molecular weight of 120kD (4). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.
Catalog #	T8050-21
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 2-4ug/1x10e6 Ramos cells for intracellular staining
	Western Blot: 1-3ug/ml
	Immunoprecipitation: 2ug/10e6 transfected cells
	Immunohistochemistry: Paraffin sections
	Optimal dilutions to be determined by the researcher.
Positive Control	Human intestine or Ramos cells (for intracellular staining)
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	4H269
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity	Purified by Protein G Chromatography.
Immunogen	Synthetic peptide corresponding to aa 55-70 [VLNLTHNQLRRLPAAN] of human TLR3.
Specificity	Recognizes human TLR3 (Toll Like Receptor 3).
Species Crossreactivity	Canine
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.