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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR3 (Toll Like Receptor 3) (FITC)

Anti -TLR3 (Toll Like Receptor 3) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B FC
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 cDNA codes for a protein with approximate molecular weight of 120kD (4). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.
Catalog #T8050-21A
ApplicationsSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Flow Cytometry: 2-5ug/1x10e6 Ramos cells for intracellular staining.
Optimal dilutions to be determined by researcher.
Positive Control Human intestine tissue lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4H270
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide of human TLR3 (KLH coupled).
SpecificityRecognizes human TLR3 (Toll Like Receptor 3). Species Crossreactivity: Human. Does not crossreact with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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