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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR4 (Toll Like Receptor 4, CD284)

Anti -TLR4 (Toll Like Receptor 4, CD284)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IC FC IF N
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88kD.
Catalog #T8050-29B
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Confocal Microscopy and Neutralization Assay. Other applications not tested.
Recommended DilutionFlow Cytometry (Cell Surface): 2-3ug/1x10e6 (tested on PBMCs)
Immunoprecipitation: see ref 8 for details
FA(Neutralization): see ref 9 for details.
Immunofuorescence/Immunocytochemistry: see ref 10 for details
Optimal dilution to be determined by the researcher.
Recommended Positive Control PBMCs or ThP1
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No4H272
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein G chromatography
ImmunogenBa/F3 cell line expressing human TLR4 cell surface antigen
SpecificityRecognizes human TLR4 (Toll Like Receptor 4). Has been shown to block the activation of monocytes with LPS. Species Crossreactivity: canine and mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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