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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2) (FITC)

Anti -TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria.
Catalog #T8050-29C
TLR4/MD-2 Complex is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells.
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityFor long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9G281
HostRat
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified.
ImmunogenMouse TLR4/MD-2.
SpecificityRecognizes mouse toll-like receptor 4 (TLR4), when associated with MD-2 and co-precipitates MD-2 with TLR4. The antibody was shown to coprecipitate MD-2 (30kD) with TLR4 (100kD). It does not detect TLR4 alone.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesTRL4, CD284, lymfocyte antigen 96, ESOP-2


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