| The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main |
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| mediatiors of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kD (6). |
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| Catalog # | T8050-29H |
| Applications | Suitable for use in Western Blot, Flow cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-3ug/ml |
| Immunohistochemistry: 1:50 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies I1904-39 | IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 0.05% BSA. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa420-435 of TLR4. |
| Specificity | Recognizes human TLR4/CD284 at ~75-80kD. Species Crossreactivity: mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
