Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR4 (Toll-like Receptor 4, CD284, LPS)

Anti -TLR4 (Toll-like Receptor 4, CD284, LPS)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
TLR4, a type I membrane protein that belongs to the Toll-like receptor family, cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response TLR4 Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR. TLR4 binds to LY96 via the extracellular domain, and to MyD88 and TIRAP via their respective TIR domains. The protein contains 19 leucine-rich (LRR) repeats, and It is highly expressed in heart, spleen, lung and muscle. Lower levels are found in liver and kidney. Interstrain analyses reveal that TLR4 is a polymorphic protein and that the extracellular domain is far more variable than the cytoplasmic domain, which is variable at the C-terminal.
Catalog #T8050-29L
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No76B357.1
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa100-200 of human TLR4 (KLH).
SpecificityRecognizes human TLR4. Species sequence homology: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links