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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR4/MD-2 Complex, TLR4 (Toll Like Receptor 4/MD-2, Toll Like Receptor 4)

Anti -TLR4/MD-2 Complex, TLR4 (Toll Like Receptor 4/MD-2, Toll Like Receptor 4)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IP FC
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90kD (6).
Catalog #T8050-30
ApplicationsSuitable for use in Immunoprecipitation(1), Flow Cytometry, Functional Studies and Immunoblot(7). Other applications not tested.
Recommended DilutionFlow Cytometry: 2-5ug per1 x 10e6.
Immunoblot: Reported to be suitable for use in Immunoblot by an independent lab.
Optimal dilutions to be determined by researcher.
Positive ControlRaw cells or thioglycolate-elicited mouse macrophage cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No4H273
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenBa/F3 cell line expressing mouse TLR4 and MD-2
SpecificityRecognizes mouse TLR4 (Toll Like Receptor 4). Preferentially recognizes TLR4-MD-2 complex than of TLR4 alone. Blocks activation of monocytes with LPS (7).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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