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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR4/MD-2 Complex, TLR4 (Toll Like Receptor 4/MD-2, Toll Like Receptor 4) (Azide Free)

Anti -TLR4/MD-2 Complex, TLR4 (Toll Like Receptor 4/MD-2, Toll Like Receptor 4) (Azide
Free)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B FC
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88kD (6).
Catalog #T8050-30D
ApplicationsSuitable for use in Flow Cytometry and FA. Other applications have not been tested.
Recommended DilutionFlow Cytometry: 2-5 ug/10e6 cells. However, the optimal condition has to be determined for individual experiments.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No4H273
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS. No preservatives added.
PurityPurified by Protein G chromatography
ImmunogenBa/F3 cell line expressing mouse TLR4 and MD-2
SpecificityRecognizes mouse TLR4 (Toll Like Receptor 4). Preferentially recognizes TLR4-MD-2 complex than of TLR4 alone.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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