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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, CD284, CD284 Antigen, Homolog of Drosophila Toll, hToll, Toll (Drosophila) Homolog, TOLL, Toll Endotoxin Hyporesponsiveness)

Anti -TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, CD284, CD284
Antigen, Homolog of Drosophila Toll, hToll, Toll (Drosophila) Homolog, TOLL, Toll
Endotoxin Hyporesponsiveness)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC
TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Catalog #T8050-31F
ApplicationsSuitable for use in Flow Cytometry, Functional Assays, and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10B1271
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS. No preservative added.
PurityPurified by Protein G affinity chromatography.
ImmunogenBa/F3 cell line expressing TLR4.
SpecificityRecognizes the human Toll like receptor 4 (TLR4). Species Crossreactivity: Rhesus Monkey, Guinea Pig, Porcine, Canine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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