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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, CD284, CD284 Antigen, Homolog of Drosophila Toll, hToll, Toll (Drosophila) Homolog, TOLL, Toll Endotoxin Hyporesponsiveness)

Anti -TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, CD284, CD284
Antigen, Homolog of Drosophila Toll, hToll, Toll (Drosophila) Homolog, TOLL, Toll
Endotoxin Hyporesponsiveness)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IP FC
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90kD (6).
Catalog #T8050-33F
ApplicationsSuitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Positive Control RAW or thioglycolate-elicited mouse macrophage cells
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No9F253
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenThis antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).
SpecificityIn FA, the antibody blocks activation of monocytes with LPS (Akashi et al. 2000). This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone. The optimal condition has to be determined for individual experiments. Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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