| TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. |
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| TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS. Removal of sodium azide is recommended prior to use in functional assays. |
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| Catalog # | T8050-34 |
| Applications | Suitable for use in Immunoprecipitation and Flow Cytometry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:5-1:12.5; Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. |
| Optimal working dilutions to be determined by researcher. |
| Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. |
| Recommended Secondary Reagents | I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo |
| I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo |
| Recommended Negative Controls | I1904-83P1: Mouse IgG2a Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3F5 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Ba/F3 cell line expressing TLR4 |
| Specificity | Recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. Species Crossreactivity: Rhesus monkey, porcine, canine and guinea pig. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
