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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR4 (Toll Like Receptor 4, CD284)

Anti -TLR4 (Toll Like Receptor 4, CD284)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP FC
TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.
TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS. Removal of sodium azide is recommended prior to use in functional assays.
Catalog #T8050-34
ApplicationsSuitable for use in Immunoprecipitation and Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:5-1:12.5; Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.
Optimal working dilutions to be determined by researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Recommended Secondary ReagentsI1904-66A: IgG, F(abí)2 (FITC) Pab Rb xMo
I1904-19C: IgG, F(abí)2 (HRP) Pab Gt xMo
Recommended Negative ControlsI1904-83P1: Mouse IgG2a Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F5
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenBa/F3 cell line expressing TLR4
SpecificityRecognizes the human Toll like receptor 4 (TLR4) cell surface antigen. Species Crossreactivity: Rhesus monkey, porcine, canine and guinea pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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