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Abs to Toll Like Receptors (TLR)
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Anti -TLR4 (Toll Like Receptor 4, CD284) (FITC)
Anti -TLR4 (Toll Like Receptor 4, CD284) (FITC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC |
|
| TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 mediates the innate immune response to bacterial lipopolysaccharide. | | | Catalog # | T8050-34C | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood. | | Optimal dilutions to be determined by the researcher. | | Recommended Negative Control | I1904-83Y1: IgG2a Negative Control (FITC) | | | | Hybridoma: | | Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 3F5 | | Host | Mouse | | Source | Human | | Concentration | ~0.05mg/ml | | Form | Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide before the addition of glycerol to 40%. Conjugated to FITC. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Ba/F3 cell line expressing TLR4 | | Specificity | Recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. Species Crossreactivity: Rhesus monkey | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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