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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR4 (Toll Like Receptor 4, CD284) (PE)

Anti -TLR4 (Toll Like Receptor 4, CD284) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 mediates the innate immune response to bacterial lipopolysaccharide.
Catalog #T8050-34D
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat. Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.
Optimal dilutions to be determined by the researcher.
Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F5
HostMouse
SourceHuman
Concentration~100 test/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, 1% BSA, 40% glycerol.
PurityPurified by Protein G affinity chromatography.
ImmunogenBa/F3 cell line expressing TLR4.
SpecificityRecognizes the human Toll like receptor 4 (TLR4) cell surface antigen. Species Crossreactivity: Monkey, Rhesus monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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