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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR5 (Toll-like Receptor 5, Toll/Interleukin-1 Receptor-like Protein 3, TIL3) (FITC)

Anti -TLR5 (Toll-like Receptor 5, Toll/Interleukin-1 Receptor-like Protein 3, TIL3) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.
Catalog #T8050-41B
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry (Intracellular): 0.5-1ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No11E374
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 10mM Tris, 150mM sodium chloride, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to a portion of human TLR5 between aa700-800 conjugated to KLH (Genbank #NP_003259).
SpecificityRecognizes an intracellular epitope in the cytoplasmic domain of human TLR5. Species Crossreactivity: mouse and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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