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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

TLR5, a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents. It also plays a role in mediating detection of bacterial flagellins. TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues. The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella. Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA). The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.
Catalog #	T8050-45F
Applications	Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:1,000
	Western Blot: 1:100-1:500
	Immunohistochemistry: 1:50-1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Synthetic peptide selected from the C-terminal region of mouse TLR5 (KLH).
Specificity	Recognizes mouse TLR5.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.