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You are here:Home » Antibodies » Abs to Toll Like Receptors (TLR) » Anti -TLR9 (Toll Like Receptor 9, CD289) (PE)

Anti -TLR9 (Toll Like Receptor 9, CD289) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, Hemmi et al., 2000 have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TRL9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen.
Catalog #T8050-94
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Reported by an independent laboratory to be suitable for Confocal Microscopy. Other applications not tested.
Recommended DilutionsFlow Cytometry (Intracellular): 0.1-0.5ug/1x10e6 cells
Immunohystochemistry (Frozen): 10-20ug/ml
Immunohistochemistry (Paraffin): Reported by an independent laboratory to be suitable for use on paraffin sections.
Optimal dilutions to be determined by the researcher.
Positive ControlRamos, human PBMCs
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No4H286
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa268-284, CPRHFPQLHPDTFSHLS of human TRL9, conjugated to KLH.
SpecificityRecognizes human TLR9 (Toll Like Receptor 9). Species Crossreactivity: mouse, rat, canine, equine and Rhesus monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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