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Anti -TLR9 (Toll Like Receptor 9, CD289) (PE)
Anti -TLR9 (Toll Like Receptor 9, CD289) (PE)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH FC |
|
| The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. By using a BLAST search, Hemmi et al., 2000 have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TRL9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA. Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen. | | | Catalog # | T8050-94 | | Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. Reported by an independent laboratory to be suitable for Confocal Microscopy. Other applications not tested. | | Recommended Dilutions | Flow Cytometry (Intracellular): 0.1-0.5ug/1x10e6 cells | | Immunohystochemistry (Frozen): 10-20ug/ml | | Immunohistochemistry (Paraffin): Reported by an independent laboratory to be suitable for use on paraffin sections. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Ramos, human PBMCs | | Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 4H286 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE). | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa268-284, CPRHFPQLHPDTFSHLS of human TRL9, conjugated to KLH. | | Specificity | Recognizes human TLR9 (Toll Like Receptor 9). Species Crossreactivity: mouse, rat, canine, equine and Rhesus monkey. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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