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You are here:Home » Antibodies » Abs to T Cells, Lymphocytes » Anti -T Lymphocytes

Anti -T Lymphocytes

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IP IH FC
Catalog #T8060-01
Recommended Secondary Reagents F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate
Fusion Partners Spleen cells from immunized BALB/c mice were fused with cells of the PAI mouse myeloma cell line.
ApplicationsFlow Cytometry: Neat
Immunoprecipitation
Immunohistochemistry: (frozen)- Neat
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H3061
HostMouse
SourceRabbit
ConcentrationAs reported
FormSupplied as a liquid in 0.1M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.1% sodium azide.
PurityTissue Culture Supernatant.
ImmunogenRabbit Thymocytes.
SpecificityReacts with T cells. Recognizes >90% thymocytes and 40-45% mesenteric lymph node cells. The antibody immunoprecipitates a 67kD protein from thymocytes. Little evidence of B-cell activity. This antibody was initially reported as recognizing rabbit CD5 (1), but does not bind to rabbit CD5 transfectants. Known rabbit CD5 antibodies also show binding to most B lymphocytes (2), which are not labelled by this clone.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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