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You are here:Home » Antibodies » Abs to Topoisomerases » Anti -Topoisomerase II alpha DNA

Anti -Topoisomerase II alpha DNA

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified E B IP IH IF
Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2-& M-phases in transformed and developmentally regulated normal cells. Note that Ki67/M1B1 is also present in G1-phase. Topo IIa is also implicated in drug resistance of tumor cells.
Catalog #T8065-10
ApplicationsSuitable for use in Immunoprecipitation, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (use Protein A): 10ug/mg protein lysate
Western Blot: 5-10ug/ml for 2 hrs at RT.
Optimal dilutions to be determined by the researcher.
Positive ControlRaji cells or exponentially growing any cultured human cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA, before the addition of glycerol to 40%. Also supplied without BSA and Sodium Azide. See T8065-10X.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide, corresponding to aa 1513-1530 (Cys-RAKKPIKYLEESDEDDLF) from the C-terminus of human topoisomerase IIa (KLH). Cellular Localization
SpecificityRecognizes a 170kD protein, which is identified as topoisomerase IIa. It shows no crossreaction with Topoisomerase IIb or I. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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