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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E B IP IH IF

Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2-& M-phases in transformed and developmentally regulated normal cells. Note that Ki67/M1B1 is also present in G1-phase. Topo IIa is also implicated in drug resistance of tumor cells.
Catalog #	T8065-10
Applications	Suitable for use in Immunoprecipitation, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunoprecipitation (use Protein A): 10ug/mg protein lysate
	Western Blot: 5-10ug/ml for 2 hrs at RT.
	Optimal dilutions to be determined by the researcher.
Positive Control	Raji cells or exponentially growing any cultured human cells.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA, before the addition of glycerol to 40%. Also supplied without BSA and Sodium Azide. See T8065-10X.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide, corresponding to aa 1513-1530 (Cys-RAKKPIKYLEESDEDDLF) from the C-terminus of human topoisomerase IIa (KLH). Cellular Localization
Specificity	Recognizes a 170kD protein, which is identified as topoisomerase IIa. It shows no crossreaction with Topoisomerase IIb or I. Species Crossreactivity: human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.