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You are here:Home » Antibodies » Antibodies-Topoisomerases » Anti -Topoisomerase II (DNA) alpha

Anti -Topoisomerase II (DNA) alpha


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Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH IC FC
The expression of topoisomerase II alpha is strongly restricted to proliferating cells. The topoisomerase II alpha antigen is preferentially expressed during G1, S, G2 and M phase of the cell cycle, while resting, non-cycling cells (G0 phase) lack topoisomerase II alpha. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase II alpha during the entire cell-cycle. This specificity for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas and gangliomas.
Catalog #T8065-11
ApplicationsSuitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-10ug/ml
Immunohistochemistry: 5-10ug/ml. Strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections.
Immunocytochemistry: 5-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No2Q382
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
ImmunogenNuclear protein preparation from the human cell line U937.50
SpecificityRecognizes a major protein of 170kD which was identified as the human alpha isoform of topoisomerase II. Recognizes a C- terminal alpha-isoenzyme specific epitope missing in topoisomerase II beta.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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