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You are here:Home » Antibodies » Abs to Topoisomerases » Anti -TOP2A (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha Isozyme, TOP2)

Anti -TOP2A (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha Isozyme, TOP2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22.The TOP2A gene spans approximately 30kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II2.
Catalog #T8065-12G
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot:1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide mapping at the C-terminal of human TOP2A, different from the related mouse sequence by 4 aa.
SpecificityRecognizes human TOP2A. Species Cross-Reactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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