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You are here:Home » Antibodies » Abs to Topoisomerases » Anti -TOP2A (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha Isozyme, TOP2)

Anti -TOP2A (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha Isozyme, TOP2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
DNA topoisomerase 2-alpha is a 170kD member of the type II topoisomerase family. Human TOP2A is 1531aa in length, and splicing variants produce three additional isoforms which have substitutions following Lys321, Gln355, or Ala401. TOP2A contains a histidine kinase-like ATPases domain (aa80-193), a TopoIIA Trans ScTopoIIA domain (aa265-417), a TOPRIM TopoIIA: topoisomerase-primase domain (aa455-575), a DNA Topoisomerase IV domain (aa697-1169), and a NES (aa1018-1028). There are multiple potential Ser/Thr phosphorylation sites. Human TOP2A shares 89aa sequence identity with mouse TOP2A.
Catalog #T8065-12H1
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2A
Clone No11n196
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to aa1174-1531 from human TOP2A expressed in E. Coli (P11388).
SpecificityRecognizes human TOP2A. No cross-reactivity with recombinant TOP2B from human or mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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