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Anti -Topoisomerase II alpha (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha Isozyme, TOP2A, TOP2)
Anti -Topoisomerase II alpha (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha
Isozyme, TOP2A, TOP2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IF |
|
| Topo II-a plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Note that Ki67/M1B1 is also present in G1-phase. Topo II-a is also implicated in drug resistance of tumor cells. | | | Catalog # | T8065-12J | | Applications | Suitable for use in Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested. | | Recommended Dilution | Western Blot: 5-10ug/ml for 2h at RT | | Immunoprecipitation: 10ug/mg protein lysate | | Optimal dilutions to be determined by the researcher. | | Positive Control | Raji cells or exponentially growing any cultured human cells | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide corresponding to aa1513-1530 from the C-terminus of human topoisomerase II-a. | | Specificity | Recognizes human Topoisomerase II alpha. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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