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You are here:Home » Antibodies » Abs to Topoisomerases » Anti -Topoisomerase II alpha (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha Isozyme, TOP2A, TOP2)

Anti -Topoisomerase II alpha (DNA Topoisomerase 2-alpha, DNA Topoisomerase II, alpha
Isozyme, TOP2A, TOP2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IF
Topo II-a plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Note that Ki67/M1B1 is also present in G1-phase. Topo II-a is also implicated in drug resistance of tumor cells.
Catalog #T8065-12J
ApplicationsSuitable for use in Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot: 5-10ug/ml for 2h at RT
Immunoprecipitation: 10ug/mg protein lysate
Optimal dilutions to be determined by the researcher.
Positive ControlRaji cells or exponentially growing any cultured human cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa1513-1530 from the C-terminus of human topoisomerase II-a.
SpecificityRecognizes human Topoisomerase II alpha.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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