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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant B IP IH

DNA topoisomerase II alpha (Topo II alpha) is a 170kDa ubiquitous nuclear enzyme belonging to the topo family, which regulates the topological states of DNA. Topo II is required in chromatin condensation and segregation during mitosis. Topo II alpha is cell cycle regulated and its level peaks between G2 to M phase. It has been linked to cell proliferation and it may be the main isoform of Topo II involved mitotic processes (1). Topo II alpha passes one strand of DBA through a reversible break in a second DNA strand, which catalyzes the topological isomerisation of DNA during cell cycle (2). Topo II alpha overexpression has been linked to a number of human maligenacies and is the target for many chemotherapeutic agents. The majority of anticancer drugs targeting Topo II alpha initiate apoptosis by stabilizing the convalent complex formed between DNA and Topo II alpha (3).
Catalog #	T8065-15
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:10,000
	Immunohistochemistry: 1:100-1:250
	Immunoprecipitation: 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E592
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50 mM Tris- Glycine (pH 7.4), 0.18 Msodium chloride, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.
Purity	Supernatant
Immunogen	A synthetic peptide corresponding to C-terminal residues of human Topo II .
Specificity	Recognizes human Topoisomerase II alpha. Species crossreactivity: mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	DNA topoisomerase II, alpha isozyme