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You are here:Home » Antibodies » Abs to Topoisomerases » Anti -Topoisomerase II alpha, phosphorylated (Ser1106)

Anti -Topoisomerase II alpha, phosphorylated (Ser1106)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC
DNA topoisomerase II alpha (Topo II ) is a 170kDa ubiquitous nuclear enzyme belonging to the topo family, which regulates the topological states of DNA. Topo II is required in chromatin condensation and segregation during mitosis. Topo II is cell cycle regulated and its level peaks between G2 to M phase. It has been linked to cell proliferation and it may be the main isoform of Topo II involved mitotic processes (1). Topo II passes one strand of DBA through a reversible break in a second DNA strand, which catalyzes the topological isomerisation of DNA during cell cycle (2). Topo II overexpression has been linked to a number of human maligenacies and is the target for many chemotherapeutic agents. The majority of anticancer drugs targeting Topo II initiate apoptosis by stabilizing the convalent complex formed between DNA and Topo II (3). Results have shown esults demonstrate that Ca(2+)-regulated phosphorylation of Ser-1106 in the catalytic domain of topo II alpha modulates the enzymatic activity of this protein and sensitivity to topo II-targeting drugs (4).
Catalog #T8065-16
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1:500
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E594
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
PuritySupernatant
ImmunogenA phospho specific peptide corresponding to residues surrounding Serine 1106 of human Topoisormera II alpha.
SpecificityRecognizes human Topoisomerase II alpha phosphorylated on Serine 1106.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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