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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IF

Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates and completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil, or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Traditionally, Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.
Catalog #	T8075-40
Applications	Suitable for use in IFA and ELISA. Suitability for Immuohistochemistry and Western Blot have not been tested, but should not be excluded. Other applications have not been tested.
Recommended Dilution	IFA: 1:10-1:50
	ELISA: 1:20-1:200
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	1.B.517
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 0.01M PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Toxoplasma gondii
Specificity	Recognizes 2 polypeptides of ~24kD and 40kD. Non-reactive with cyst form.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.