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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E IF

Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates. It completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Traditionally, Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild. Ssevere infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.
Catalog #	T8076-17
Applications	Suitable for use in ELISA and IFA. Other applications not tested.
Recommended Dilution	IFA (Direct): 1:10-1:50
	Note: Acetone fixation of the antigen source is recommended prior to staining.
	Optimal dilution to be determined by researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~4-5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity	Purified by Protein A affinity chromatography (95%).
Immunogen	T. gondii RH strain purified trophozoites.
Specificity	Toxoplasma gondii trophozoites. Crossreactivity to tachyzoites has not been tested.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.