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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IF

Toxoplasma is a crescent shaped sporozoan that lives as intracellular parasite in various tissues of vertebrates and completes its life cycle in a single host. It is transmitted via raw/undercooked meat, contaminated soil, or by direct contact. Infection due to Toxoplasma gondii usually occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy following organ transplant. Traditionally, Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.
Catalog #	T8076-18
Applications	Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution	Immunofluorescence: 1:5-1:20. Acetone fixation of the antigen source is recommended prior to staining.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2
Clone No	1.B.514
Host	Mouse
Concentration	0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Conjugated with high purity isomer I of fluorescein isothiocyanate. Free fluorescein has been removed.
Purity	Purified IgG fraction
Immunogen	Toxoplasma gondii
Specificity	Specific for a 30kD membrane protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.