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T8100-05C Rabbit Anti-TPX2 (Targeting Protein for XKLP2, TPX2 Microtubule-associated Homolog, TPX2 Microtubule-associated Protein Homolog, C20orf2, Chromosome 20 Open Reading Frame 1, C20ORF1, Differentially Expressed in Cancerous and Noncancerous Lung Cells 2, Differentially Expressed in Lung Cells 2, DIL2, DIL-2, Differentially Expressed in Lung Cells, FLS353, GD:C20orf1, HCTP4, Hepatocellular Carcinoma-associated Antigen 519, HCA519, p100, Preferentially Expressed in Colorectal Cancer, Protein FLS353, REPP86, Restricted Expression Proliferation-associated Protein 100) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q9ULW0
Isotype
IgG
Grade
Serum
Applications
FC IC IF IP WB
Crossreactivity
Hu Ma Mo Po Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

TPX2 is a microtubule cross-linking protein involved in spindle formation. During mitosis it is strictly associated with the mitotic spindle, whereas during phases S and G2, it is diffusely distributed throughout the nucleus.

Applications
Suitable for use in Western Blot, Flow Cytometry Immunofluorescence/Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 detects a band at ~100kD Immunofluorescence (IC): 1:1000 Flow Cytometry: 1:1000 Immunoprecipitation: 1:10-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant segment of the C-terminal domain of human TPX2. Cellular Localization: Nuclear. Staining of non-mitotic cells and cell cycle-dependent changes in abundance peaking at G2/M phase.
Form
Supplied as a liquid. No preservative added.
Purity
Serum
Specificity
Rcognizes human TPX2. Species Crossreactivity: mammal, mouse, rat and porcine
References
1. Heidebrecht H.J. et al. p100: a novel proliferation-associated nuclear protein specifically restricted to cell cycle phases S, G2, and M. Blood. 90: 226-233(1997). 2. Garrett, S. et al. hTPX2 is required for normal spindle morphology and centrosome integrity during vertebrate cell division. Curr. Biol. 12: 2055-2059 (2002)
USBio References
No references available
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