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You are here:Home » Antibodies » Antibodies-Stem Cell Research » Anti -TRA-1-60 (TRA-1, Tumor Rejection Antigen 1) (PE)

Anti -TRA-1-60 (TRA-1, Tumor Rejection Antigen 1) (PE)


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Clone Host Grade Applications
Monoclonal Mouse FC
TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent
evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope. The original TRA-1-60 antibody is now known to include two distinct specificities that recognize a neuraminidase resistant epitope [TRA-1-60(R)] or a neuraminidase-sensitive epitope [TRA-1-60(S)].
Catalog #T8115A
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Designed to quantitatively determine the percentage of cells bearing TRA-1-60(R) within a population and qualitatively determine the density of TRA-1-60(R) on cell surfaces by flow cytometry. Cell surface expression of TRA-1-60(R) is determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector BG01V cells were stained with PE-conjugated anti-human TRA-1-6 (filled histogram) or isotype control (open histogram).
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9H185
FormSupplied as a lyophilized powder in 25ug antibody, 1ml saline, 0.5% BSA, 0.1% sodium azide.
PurityHuman embryonal carcinoma cell line, 2102E9
ImmunogenRecognizes human TRA-1-60 (PE).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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