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Anti -TRA-1-60 (TRA-1, Tumor Rejection Antigen 1) (PE)
Anti -TRA-1-60 (TRA-1, Tumor Rejection Antigen 1) (PE)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
|
FC |
|
| TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent | | | evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope. The original TRA-1-60 antibody is now known to include two distinct specificities that recognize a neuraminidase resistant epitope [TRA-1-60(R)] or a neuraminidase-sensitive epitope [TRA-1-60(S)]. | | | Catalog # | T8115A | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: Designed to quantitatively determine the percentage of cells bearing TRA-1-60(R) within a population and qualitatively determine the density of TRA-1-60(R) on cell surfaces by flow cytometry. Cell surface expression of TRA-1-60(R) is determined by flow cytometry using 488 nm wavelength excitation and monitoring emitted fluorescence with a detector BG01V cells were stained with PE-conjugated anti-human TRA-1-6 (filled histogram) or isotype control (open histogram). | | Optimal dilutions to be determined by the researcher. | | | | Storage and Stability: | | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgM | | Clone No | 9H185 | | Host | Mouse | | Source | Human | | Concentration | ~25ug/ml | | Form | Supplied as a lyophilized powder in 25ug antibody, 1ml saline, 0.5% BSA, 0.1% sodium azide. | | Purity | Human embryonal carcinoma cell line, 2102E9 | | Immunogen | Recognizes human TRA-1-60 (PE). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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