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You are here:Home » Molecular Biology » Growth Factors-Apoptosis » TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)

TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)

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Specifications

Recombinant Human soluble Trail also called TNF-related apoptosis-inducing Ligand, TNFSF10, Apo2 Ligand AND TL2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 168 amino acids and having a molecular mass of 19.6kD. TRAIL is purified by proprietary chromatographic techniques.
Catalog #T8180-15
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Arg-Glu-Arg-Gly-Pro.
Biological ActivityRecombinant Human TRAIL is fully biologically active when compared to standard. The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensetive U343MG cells, ED50 for this effect is 1-3 ng/ml.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight19.6kD
SourceE. coli
Purity97% (RP-HPLC, SDS-PAGE)
Concentration~1mg/ml
FormSupplied as a lyophilized powder from 150mM sodium chloride, 50mM sodium phosphate, pH 7. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to >0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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