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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Transferrin, also known as serotransferrin and siderophilin, is an 80kD secreted glycoprotein that binds two Fe (3+) ions, transporting them from sites of absorption and heme degradation to those of storage and utilization. It is a member of the Transferrin family of proteins. Human Transferrin is synthesized as a 698 amino acid (aa) precursor that contains a 19aa signal sequence and a 679aa mature chain. The mature chain consists of two Transferrin-like domains, two high-affinity iron binding sites, one potential site for O-linked glycosylation, and two potential sites for N-linked glycosylation. Human Transferrin has sequence homology of 73% to mouse and rat Transferrin.
Catalog #	T8199-01B
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Transferrin under non-reducing conditions only. Lysates were prepared from human liver tissue. PVDF membrane was probed with 2ug/ml of T8199-01B followed by HRP­conjugated Anti­Mouse IgG secondary antibody. A specific band was detected for Transferrin at ~90kD. Use of this antibody under reducing conditions is not recommended.
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1904-75E: IgG1 (HRP) Pab Rb x Mo
	I1904-79M: IgG1 (HRP) Pab Gt x Mo
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9H186
Host	Mouse
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G immunoaffinity chromatography.
Immunogen	Recombinant corresponding to aa20-698 of human Transferrin expressed in SF 21 insect cells (AAH59367).
Specificity	Recognizes human Transferrin in direct ELISAs and Western Blots.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.