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You are here:Home » Antibodies » Abs to Transferrin » Anti -Transferrin (NT)

Anti -Transferrin (NT)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH IF
Transferrin is a single polypeptide chain glycoprotein. It is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.
Catalog #T8199-02
ApplicationsSuitable for use in ELISA, Indirect Immuno uorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: Fixed, paraf n embedded human brain tissue.
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
SourceAscites
Affinity Constant2.85x10e-11M
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No1.B.799
HostMouse
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography. 95% (SDS-PAGE).
ImmunogenPuri ed transferrin from pooled human plasma.
SpecificityRecognizes the N-terminal domain of human transferrin. Does not crossreact with mouse, rabbit, bovine transferrin or human lactoferrin. Inhibits proliferation of Con-A stimulated peripheral blood lymphocytes (10e-6M)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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