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You are here:Home » Antibodies » Abs to Transferrin » Anti -Transferrin (Transferin, TF, Apotransferrin, Beta-1 Metal-binding Globulin, DKFZp781D0156, PRO1400, PRO1557, PRO2086, Serotransferrin Precursor, Siderophilin)

Anti -Transferrin (Transferin, TF, Apotransferrin, Beta-1 Metal-binding Globulin,
DKFZp781D0156, PRO1400, PRO1557, PRO2086, Serotransferrin Precursor, Siderophilin)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1,800–2,700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.
Catalog #T8199-03
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Functional Assays. Other applications not tested.
Recommended DilutionELISA: 1:500- 1:5000.
Immunohistochemistry (paraffin): 10ug/ml. Requires antigen retrieval using heat treatment prior to staining of paraffin sections using sodium citrate buffer, pH 6.0.
Functional Assays: Removal of sodium azide is recommended.
Optimal dilutions to be determined by the researcher.
Positive Control TissuePlacental villi
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B2492
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman transferrin
SpecificityRecognizes N-terminal domain of human transferrin. Inhibits the porliferation of Con-A stimulated blood lymphocytes in functional assays. Does not react with mouse, rabbit and bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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