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You are here:Home » Antibodies » Abs to Transferrin » Anti -Transferrin (Tf)

Anti -Transferrin (Tf)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Highly Purified Gel
Transferrin is a single polypeptide chain glycoprotein. It is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.
Catalog #T8199-04A
ApplicationsSuitable for use in Immunodiffusion. Other applications not tested.
Recommended DilutionImmunodiffusion: neat
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-63 IgG, H&L (HRP) Pab DkxSh
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in GBS, pH 7.4, 0.1% sodium azide, 0.1% EACA, 0.01% Benzamidine, 1mM EDTA.
PurityPurified by ion exchange chromatography.
ImmunogenHuman holo-transferrin.
SpecificityRecognizes human transferrin, an iron binding glycoprotein of 79kD. Species Crossreactivity: Feline, mouse, rat, rabbit, guinea pig. Does not crossreact with chicken, bovine, equine, goat, canine or porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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