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Specifications

Clone Host Grade Applications Polyclonal Sheep Highly Purified E Gel

Transferrin is a single polypeptide chain glycoprotein. It is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.
Catalog #	T8199-08
Applications	Suitable for use in ELISA, IEP, 2D-IEP, Ouchterlony. Other applications not tested.
Recommended Dilution	EIA: 1:50,000-1:100,000 (2ug/ml transferrin and TMB as a substrate).
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Sheep
Source	Human
Concentration	~5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.05% Proclin, 300, 0.002% methylisothiazolone, 0.002% bromonitrodioxane. Labeled with HRP.
Purity	Purified by Ion Exchange chromatography.
Immunogen	Human holo-transferrin purified from serum, shown to be homogeneous by SDS-PAGE.
Specificity	Recognizes transferrin. Single precipitin arc when tested by IEP and 2D-IEP against human serum. Identity has been confirmed by double diffusion (Ouchterlony) against human serum and an anti-human transferrin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.