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You are here:Home » Antibodies » Abs to Transferrin » Anti -Transferrin Receptor (TfR)

Anti -Transferrin Receptor (TfR)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IC IF
The human transferrin receptor (TfR) is a homodimeric type II membrane protein consisting of two identical 95kD subunits covalently linked by intermolecular disulfide bonds. The TfR has an extracellular domain of 671 amino acids, a single 28 residue transmembrane region and a 61 residue amino terminal cytoplasmic domain. Expression of the receptor is limited on normal tissue, however, highly expressed on the surface of some tumors. TfR is included in the class of ligand transport receptors which are internalized and clustered in coated pits. An important function of the receptor is to bind the serum transport protein transferrin (Tf) and mediate the uptake of iron into the cell. A growing area of research involves the use of monoclonal anti-TF receptor antibodies to inhibit the uptake of iron and thus block tumor cell growth.
Catalog #T8199-54
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunodepletion, Electron Microscopy, Immunogold-labeling and Iron uptake research. Other applications have not been tested.
Recommended DilutionsELISA: 0.1-1ug/ml
Western Blot: 1ug/ml
Immunoprecipitation: 2-5ug/IP rxn
Immunohistochemistry (fixed, paraffin-embedded tissue): 1-5ug/ml
Immunofluorescence: 1-5ug/ml
Immunocytochemistry: 1-5ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A202
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein corresponding to human transferrin receptor.
Species Sequence HomologyChicken, mouse, rat, Chinese hamster. Exhibits crossreactivity due to conserved amino acid sequences in the cytoplasmic tail.
SpecificityRecognizes aa3-28 of the human transferrin receptor (TfR) tail.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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