| TGF-b1, -2 and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-b1, -2 and -3 are disulfide-linked homodimers. |
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| Catalog # | T8250-16 |
| Applications | Suitable for use in capture ELISA, Neutralization, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | Capture ELISA: 2-8ug/ml. Can be paired with T8250-16B (0.1-0.4ug/ml) as the detection antibody in sandwich ELISA. |
| Neutralization: 0.3-1ug/ml will neutralize 50% of the bioactivity in the presence of 0.25ng/ml of recombinant human TGFb1 and 7.5ng/ml recombinant mouse IL-4. Measured by its ability to neutralize TGF-b1 inhibition of IL-4 dependent proliferation in the HT-2 mouse T cell line. |
| Western Blot: 1ug/ml. Reducing conditions are not recommended. |
| Flow Cytometry (Intracellular): 2.5ug/ml labels 1x10e6 cells. Validated using PC-3 human prostate cancer cell line fixed with paraformaldehyde and permeablized with saporin. |
| Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin embedded sections of human prostate cancer tissue. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo |
| I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt x Mo |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Ar2 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Endotoxin | <0.1EU/ug (LAL) |
| Immunogen | Recombinant protein corresponding to human transforming growth factor beta 1 (TGF-b1) and latent TGF-b1 expressed in CHO cells (P01137). |
| Specificity | Recognizes human TGF-b1 both in its active form and in its latent form when it is complexed with LAP (latency associated peptide.). Neutralizes the biological activity of TGF-b1. Species Crossreactivity: mouse and rat. In sandwich ELISA, < 2% crossreactivity with recombinant human TGF-b3 and recombinant amphibian TGF-b5 was observed and no crossreactivity with recombinant porcine or recombinant human TGFb2 is observed. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
