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You are here:Home » Growth Factors, Cytokines » Growth Factors-TGF » Transforming Growth Factor beta 3, Recombinant, Human (TGFb3)

Transforming Growth Factor beta 3, Recombinant, Human (TGFb3)

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Specifications

Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
Catalog #T8250-45
T8250-45 is produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 112aa and having a molecular mass of 25.45kD
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.
Biological ActivityThe ED50 as determined by the cell toxicity assay using the WHO Standard 98/608 as a direct comparison is <0.05ng/ml, corresponding to a Specific Activity of 20MU/mg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TGF-beta 3 as a Reference Standard.
Storage and StabilityMay be stored at -20°C. For long-term storage, add 0.1% HSA or BSA, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight25.45kD
SourceE. coli
Purity~98% as determined by RP-HPLC and SDS-PAGE.
Concentration~0.25mg/ml
FormSupplied as a liquid in 20% ethanol and 0.1% acetate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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