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You are here:Home » Growth Factors, Cytokines » Growth Factors-TGF » Transforming Growth Factor beta2, Recombinant, Human (TGF b2)

Transforming Growth Factor beta2, Recombinant, Human (TGF b2)

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Specifications

The three mammalian isoforms of TGF-beta, TGF-beta1, TGF-beta2, TGF-beta3, signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation, motility and synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and would healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-beta2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth. It may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development. Recombinant human TGF-beta2 is a 25kD protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
Catalog #T8251
Amino Acid SequenceALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHSR VLSLYNTINP EASASPCCVS QDLEPLTILY YIGKTPKIEQ LSNMIVKSCK CS
Biological ActivityDetermined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED50 was found to be 0.2ng/ml, corresponding to a specific activity of 5x10e6 units/mg.
Storage and Stability Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with ddH2O, 0.1% BSA . Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceBaculovirus-infected High-5 insect cells
PurityHighly purified (~98% by SDS-PAGE and HPLC)
Endotoxin0.1ng/ug (1EU/ug)
FormSupplied as a lyophilized powder from sodium citrate, pH 3.5. Reconstitute with sterile ddH2O, 0.1% BSA to 0.1-1mg/ml. Do not vortex.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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