TREM-2 is a single Ig domain receptor expressed by cells of myeloid lineage. Two closely related transcripts were reported in mouse and designated TREM-2a and TREM-2b. TREM-2b is a pattern recognition receptor that binds anionic ligands. TREM-2 associates with the adapter protein DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses.
Applications
Suitable for use in Flow Cytometry, Direct ELISA and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 2.5ug/10e6 cells using RAW 264.7 mouse monocyte/macrophage cell line, THP-1 human acute monocytic leukemia cell line, human mature dendritic cells and HEK293 human embryonic kidney cell line transfected with eGFP and either human TREM-2 or human TREM-1. Immunocytochemistry: 5-25ug/ml using immersion fixed RAW 264.7 mouse monocyte/macrophage cell line Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to mouse TREM-2b extracellular domain expressed in NS0 cells.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul or 1ml sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant
Specificity
Recognizes mouse TREM-2b. Stains TREM-2 transfectants but not TREM-1 transfectants. Species Crossreactivity: human
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