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You are here:Home » Antibodies » Abs to Tubulin » Anti -Triadin

Anti -Triadin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IP IH
The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPRís) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyRís), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, Triadin (MW 95kD) transmits electrochemical signals to the SR through direct interaction with both DHPRís and RyRís. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.
Catalog #T8320
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Detects a 95kD protein representing Triadin from rabbit skeletal muscle extracts.
Immunohistochemistry: (Frozen sections) 1:50. Immunohistochemical staining of Triadin in rat skeletal muscle results in striated staining consistent with sarcoplasmic reticulum localization.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No4i341
HostMouse
SourceRabbit
ConcentrationNot determined
FormSupplied as a liquid in PBS with 0.05% sodium azide.
PurityAscites.
ImmunogenPurified rabbit fast skeletal muscle Triadin.
SpecificityCrossreacts with Rabbit, Rat and Mouse Triadin
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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