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Trichostatin A (TSA) (Histone Deacetylase Inhibitor)
Trichostatin A (TSA) (Histone Deacetylase Inhibitor)
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| Trichostatin A is an antifungal antibiotic that is a reversible, potent and specific inhibitor of mammalian histone deacetylase (HDAC) both in vivo and in vitro. Histone deacetylase inhibition causes chromatin relaxation and gene expression modulation. May induce apoptosis. TSA inhibits the eukaryotic cell cycle and induces morphological reversion of transformed cells. It blocks cell cycle progression at G1 phase in Hela Cells. TSA causes accumulation of highly acetylated histones in vivo, while inhibiting the activity of partially purified histone deacetylase in vitro. | | | Catalog # | T8375-05 | | Applications | Suitable for use in Inhibition of Histone Deacetylation. Other applications not tested. | | Recommended Dilution | Inhibition of Histone Deacetylation: Incubation of A431 cells with 50-100ng/ml Trichostatin A for 24 hours inhibited deacetylation of histones in vivo, as detected using anti-acetylated Histone H4. | | The optimal concentration of Trichostatin A should be empirically determined for the type of cells and assay used. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 3-4 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | 58880-19-6 | | Molecular Formula | C17H22N2O3 | | Molecular Weight | 302.4 | | Source | Streptomyces hygroscopicus | | Purity | Highly Purified ( 99% by HPLC). | | Concentration | ~5mg/ml | | Form | Supplied as a liquid in methanol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | 4,6-Dimethyl-7-[p-dimethylaminophenyl]- 7-oxohepta-2, 4-dienohydroxamic acid) |
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