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You are here:Home » Antibodies » Abs to Transcription Factors, TRIM (Tripartite Motif) » Anti -TRIM (T Cell Receptor-Interacting Molecule)

Anti -TRIM (T Cell Receptor-Interacting Molecule)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B FC
Human TRIM (T cell receptor-interacting molecule) (also trat1, T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 30kD, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family. It contains a short, 8aa extracellular region, a 19aa transmembrane region, and a 159aa cytosolic tail. Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins. TRIM is present in T cells and NK cells. Human TRIM shares 66% aa sequence identity with mouse TRIM.
Catalog #T8464-35C
ApplicationsSuitable for use in Flow Cytometry, ELISA, and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 25ug/ml
ELISA: 0.5-1.0ug/ml
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H187
HostMouse
SourceHuman
Concentration~500ug/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human TRIM, aa50-186; Q6PIZ9 (E. coli).
SpecificityRecognizes human TRIM.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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