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You are here:Home » Antibodies » Abs to Transcription Factors, TRIM (Tripartite Motif) » Anti -TRIM5a, NT (Tripartite Motif Protein 5a)

Anti -TRIM5a, NT (Tripartite Motif Protein 5a)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
TRIM5a, otherwise known as RNF88 (RING finger protein 88), a cytoplasmic protein and member of the TRIM/RBCC family, known to play a vital role in species-specific early defence against retrovirus infection.Studies suggest that it is the association of TRIM5 with specific retroviral capsid sequences which determines the effectiveness of the defence against infection. For example, in Old World monkeys, TRIM5alpha specifically associates with HIV type 1 (HIV-1), whereas in humans TRIM5alpha is not effective against HIV-1, but can inhibit strains of the murine leukemia virus (MLV).
Catalog #T8465-02E
ApplicationsSuitable for use in Immunohistochemistry (paraffin), ELISA and Western Blot. Other applications no tested.
Recommended DilutionImmunohistochemistry (paraffin): 1ug/ml, requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
ELISA: 1:16,000
Western Blot: 1-3ug/ml detects a band of a~37kD in human brain cell lysates.
Optimal dilution to be determined by researcher.
Positive ControlHuman tonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.3, 0.02% sodium azide and dH2O 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenPeptide sequence ASGILVNVKEEVTC corresponding to the N-Terminal region of TRIM5 (NP_149023.1; NP_149083.1; NP_149084.1).
SpecificityRecognizes an epitope within the N-terminal (NT) region of human TRIM5 alpha.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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