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Abs to Transcription Factors, TRIM (Tripartite Motif)
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Anti -TRIM5a (Tripartite Motif Protein 5a)
Anti -TRIM5a (Tripartite Motif Protein 5a)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has six alternately spliced isoforms, the longest of which is the a variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). Expression of TRIM5a variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV- 1 (2,3), albeit at differing efficiencies. All TRIM5a variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5a acts as a natural barrier to cross-species retrovirus transmission (3). | | | Catalog # | T8465-03 | | Applications | Can be used for detection of TRIM5a by Western blot at 1-2ug/ml. Human uterus cell lysate can be used as a positive control and a band at approximately 55kD can be detected. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to amino acids near the midregion of human TRIM5a (Genbank accession No. NP_149023) | | Specificity | Recognizes human TRIM5a. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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