| Reacts specifically and rapidly with thiols to form mixed disulfides. Used to probe the structures of the ACh receptor channel of the GABAA receptor channel and of lactose permease. |
|
| Catalog # | T8488 |
| At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication). |
| For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly. |
| •DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents). |
| Melting Point | 151-158°C |
| Solubility | Methanol, Water |
| Stability | Moisture sensitive |
| Atmosphere | Inert gas |
| Method for Determining Identity | Proton NMR (D2O) Spectroscopic and Mass Spectrometric Analysis |
| TLC Conditions | C18 (Reverse Phase); Acetic Acid: Isopropanol: Water; 3:2:1; Visualized with ninhydrin; Single spot Rf=0.65. |
| Bromide Content | ~30% |
| Storage and Stability | May be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Moisture Sensitive. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Toxicity and Hazards | All products should be handled by qualified personnel only, trained in laboratory procedures. |
| CAS Number | 91774-25-3 |
| Molecular Formula | C6H16BrNO2S2 |
| Molecular Weight | 278.23 |
| Source | Synthetic |
| Purity | ~90% |
| Form | Supplied as a white solid. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | MTSET |
