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Specifications

Immobilized anhydrotrypsin selectively adsorbs peptides with Arg, Lys or AECys (S-aminoethyl cysteine) residues at the C- terminus under weak acidic conditions (1). Strongly acidic conditions are used to elute the bound peptides. Peptides with these amino acids at the N-terminus or internally, as well as free amino acids are not bound. Serine protease inhibitors such as ecotin and PAI-1 may also be purified on this resin (2). May be used repeatedly.
Catalog #	T8656-21
Source	Anhydrotrypsin, from bovine pancreas
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source	Bovine pancreas
Purity	~95% (SDS-PAGE)
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.15M sodium chloride, 0.02% sodium azide, pH 4.0.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.