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T8663 Rabbit Anti-TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Swiss Prot
P35739
Isotype
IgG
Grade
Affinity Purified
Applications
IC IHC IP WB
Crossreactivity
Hu Mo Rt
Accession #
NM_021589
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
BSA Free

TrkA has an apparent molecular weight 140kD and is a high affinity nerve growth factor (NGF).

Applications
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.5-2.0ug/ml detected TrkA in RIPA lysates from human A431 cells, TrkA in mouse 3T3 and rat PC-12 cell lysates. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with T8663 (2ug/ml). Proteins were visualized using a IgG (HRP) Gt x Rb and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates TrkA from 500ug of PC-12 RIPA lysate. Immunohistochemistry (Paraffin): 1:200 detected TrkA in neurons of the hippocampal region and adrenal gland’s medulla. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Immunocytochemistry: 10ug/ml showed positive staining for TrkA in PC-12 cells fixed with 4% paraformaldehyde. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
Included Positive Antigen Control
A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes rat TrkA, at ~140kD. Does not crossreact with TrkB or TrkC. Species Crossreactivity: human and mouse
References
1. Verhey, K.J., et al., J. Cell Biol. 152: 959-970 (2001). 2. Pahlman, S. and Hoehner, J.C., Molecular Medicine Today 2: 432 (1996). 3. Clary, D.O., et al., Molecular Bio. Cell 5: 549 (1994). 4. Clary, D.O., et al., Proc. Natl. Acad. Sci. USA. 91: 11,133 (1994).
USBio References
No references available
Conjugates
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