Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.

Western Blot: 0.5-2.0ug/ml detected TrkA in RIPA lysates from human A431 cells, TrkA in mouse 3T3 and rat PC-12 cell lysates. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with T8663 (2ug/ml). Proteins were visualized using a IgG (HRP) Gt x Rb and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates TrkA from 500ug of PC-12 RIPA lysate. Immunohistochemistry (Paraffin): 1:200 detected TrkA in neurons of the hippocampal region and adrenal gland’s medulla. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Immunocytochemistry: 10ug/ml showed positive staining for TrkA in PC-12 cells fixed with 4% paraformaldehyde. Optimal dilutions to be determined by the researcher.

I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb

A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

Purified by Protein A affinity chromatography.

Recognizes rat TrkA, at ~140kD. Does not crossreact with TrkB or TrkC. Species Crossreactivity: human and mouse