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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

Anti -TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IC
TrkA has an apparent molecular weight 140kD and is a high affinity nerve growth factor (NGF).
Catalog #T8663
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5-2.0ug/ml detected TrkA in RIPA lysates from human A431 cells, TrkA in mouse 3T3 and rat PC-12 cell lysates. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with T8663 (2ug/ml). Proteins were visualized using a Gt x Rb IgG (HRP) and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates TrkA from 500ug of PC-12 RIPA lysate.
Immunohistochemistry (Paraffin): 1:200 detected TrkA in neurons of the hippocampal region and adrenal glandís medulla.
Immunocytochemistry: 10ug/ml showed positive staining for TrkA in PC-12 cells fixed with 4% paraformaldehyde.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
Included Positive Antigen ControlA0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15 sodium chloride, 0.05% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenBacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.
SpecificityRecognizes rat TrkA, at ~140kD. Does not crossreact with TrkB or TrkC. Species Crossreactivity: human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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