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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -TrkA, phosphorylated (Tyr490) (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR, Neurotrophin Receptor, NTR)

Anti -TrkA, phosphorylated (Tyr490) (Tyrosine Kinase Receptor A, Nerve Growth Factor
Receptor, NGFR, Neurotrophin Receptor, NTR)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).
Catalog #T8663-04
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:100
Optimal dilution to be determined by the researcher.
Molecular weight 140kD
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues around Tyr490 of human TrkA. Molecular weight: 140kD.
SpecificityRecognizes endogenous levels of rat Trk only when phosphorylated at Tyr490. Can also detect the corresponding residues of phosphorylated TrkB and TrkC. Species sequence homology: human, mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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